General Information

1. Gene name: IgA1

2. Protein construct: The sequence contains the Fc region of IgA1 with an N-terminal histidine tag.

3. Source: Human

4. Expression host: HEK293 cells

QC Testing

1. Purity: >95% as determined by SDS-PAGE

2. Endotoxin: <1.0 EU per μg of protein as determined by the LAL method

3. Molecular mass: Human recombinant poly-IgA protein consists of four IgA1 Fc segments and streptavidin (SA), with a calculated molecular mass of 176 kDa.

4. Formulation: Lyophilized from sterile PBS, pH 7.4

Usage Guide

1. Stability & Storage:

Samples are shipped with ice packs at 4°C.

Samples are stable for 6 months from the date of receipt at -20°C to -80°C. After opening, samples are stable for 3 months at -20°C to -80°C.

Aliquot the protein into smaller amounts for storage.

Avoid repeated freeze-thaw cycles.

2. SDS-PAGE:

Protein Description

IgA is the most abundant immunoglobulin in the mucosal region, but is only the second most common antibody isoform in serum. In the blood, IgA interacts with an Fc receptor called FcαRI, which is expressed on immune effector cells, triggering an inflammatory response. Linking FcαRI via IgA-containing immune complexes causes antibody-dependent cell-mediated cytotoxicity. Human recombinant poly IgA is an analogue of the IgA immune complex, which has similar functions to the IgA complex in the blood, and can bind to FcαRI to induce downstream reactions.

References

Xinfang Xie et al. J Pathol. 2021 Jun. Renal deposition and clearance of recombinant poly-IgA complexes in a model of IgA nephropathy

For scientific research use only. Do not use for diagnostic, medical or other purposes.